File Name: C:\Program Files\Bio-Rad\Bio-Plex Manager 5.0\users\Craig Miller\Results\Saliva IgG Luitpold d71 070813.rbx
Analyte: IgG (77)
Acquisition Date: 08-Jul-2013, 12:25 PM
Reader Serial Number: LX10008241401
Plate ID:
RP1 PMT (Volts): 645.42
RP1 Target: 17026
Analyte	Type	Well	Outlier	Description	FI	FI - Bkgd	Std Dev	Std Err	%CV	Obs Conc	Exp Conc	(Obs/Exp) * 100	Conc in Range	Bead Count	Sampling Errors
IgG (77)	B	A1,B1,C1,D1	0		116.1	116.1	3.07	1.53	2.64
IgG (77)	S1	E1,F1	1	Standard 1	---	---					1000
IgG (77)	S2	G1,H1	0	Standard 2	16163.3	16047.1	739.99	523.25	4.58	447.63	500	90	447.63
IgG (77)	S3	A2,B2	0	Standard 3	14997.8	14881.6	1254.76	887.25	8.37	273.18	250	109	273.18
IgG (77)	S4	C2,D2	0	Standard 4	12149.3	12033.1	538.46	380.75	4.43	127.94	125	102	127.94
IgG (77)	S5	E2,F2	0	Standard 5	8436.8	8320.6	66.82	47.25	0.79	59.6	62.5	95	59.6
IgG (77)	S6	G2,H2	0	Standard 6	5574	5457.9	46.67	33	0.84	31.53	31.25	101	31.53
IgG (77)	S7	A3,B3	0	Standard 7	3332.5	3216.4	66.47	47	1.99	16.05	15.63	103	16.05
IgG (77)	S8	C3,D3	2	Standard 8	1816	1699.9	0	0	0	7.7	7.81	99	7.7
IgG (77)	X1	E3,F3	0	4661	6783.5	6667.4	91.22	64.5	1.34	4194.93			4194.93
IgG (77)	X2	G3,H3	0	4331	5460.8	5344.6	820.6	580.25	15.03	3064.1			3064.1
IgG (77)	X3	A4,B4	0	4326	5324.5	5208.4	23.33	16.5	0.44	2958.67			2958.67
IgG (77)	X4	C4,D4	0	4651	17175	17058.9	89.1	63	0.52	*100085.60			***
IgG (77)	X5	E4,F4	0	4654	7551	7434.9	161.22	114	2.14	4957.01			4957.01
IgG (77)	X6	G4,H4	0	4655	2923	2806.9	12.73	9	0.44	1364.07			1364.07
IgG (77)	X7	A5,B5	0	4659	8383.3	8267.1	76.01	53.75	0.91	5895.32			5895.32
IgG (77)	X8	C5,D5	0	4662	10952	10835.9	59.4	42	0.54	9918.54			9918.54
IgG (77)	X9	E5,F5	0	4666	6013.3	5897.1	345.42	244.25	5.74	3511.59			3511.59
IgG (77)	X10	G5,H5	0	4667	15148.3	15032.1	140.36	99.25	0.93	28806.19			28806.19
IgG (77)	X11	A6,B6	0	4670	11439	11322.9	183.85	130	1.61	10977.86			10977.86
IgG (77)	X12	C6,D6	0	4671	14963.8	14847.6	85.91	60.75	0.57	27000.59			27000.59
Analyte	Type	Well	Outlier	Description	FI	FI - Bkgd	Std Dev	Std Err	%CV	Obs Conc	Exp Conc	(Obs/Exp) * 100	Conc in Range	Bead Count	Sampling Errors
IgG (77)	B	A1	0		120.5	120.5								374
IgG (77)	B	B1	0		114	114								394
IgG (77)	B	C1	0		114	114								389
IgG (77)	B	D1	0		116	116								443
IgG (77)	S1	E1	1		18523	18406.9					1000			435
IgG (77)	S1	F1	1		18629	18512.9					1000			389
IgG (77)	S2	G1	0		16686.5	16570.4				*623.22	500	125	***	444
IgG (77)	S2	H1	0		15640	15523.9				349	500	70	349	413
IgG (77)	S3	A2	0		15885	15768.9				389.32	250	156	389.32	489
IgG (77)	S3	B2	0		14110.5	13994.4				207.36	250	83	207.36	416
IgG (77)	S4	C2	0		12530	12413.9				139.35	125	111	139.35	448
IgG (77)	S4	D2	0		11768.5	11652.4				117.75	125	94	117.75	446
IgG (77)	S5	E2	0		8389.5	8273.4				59.03	62.5	94	59.03	410
IgG (77)	S5	F2	0		8484	8367.9				60.18	62.5	96	60.18	523
IgG (77)	S6	G2	0		5541	5424.9				31.27	31.25	100	31.27	382
IgG (77)	S6	H2	0		5607	5490.9				31.79	31.25	102	31.79	381
IgG (77)	S7	A3	0		3285.5	3169.4				15.76	15.63	101	15.76	472
IgG (77)	S7	B3	0		3379.5	3263.4				16.33	15.63	105	16.33	436
IgG (77)	S8	C3	1		1748	1631.9					7.81			255
IgG (77)	S8	D3	0		1816	1699.9				7.7	7.81	99	7.7	453
IgG (77)	X1	E3	0		6719	6602.9				4134.79			4134.79	372
IgG (77)	X1	F3	0		6848	6731.9				4255.65			4255.65	406
IgG (77)	X2	G3	0		4880.5	4764.4				2627.58			2627.58	514
IgG (77)	X2	H3	0		6041	5924.9				3534.95			3534.95	386
IgG (77)	X3	A4	0		5341	5224.9				2971.34			2971.34	430
IgG (77)	X3	B4	0		5308	5191.9				2946.03			2946.03	497
IgG (77)	X4	C4	0		17112	16995.9				*92644.83			***	468
IgG (77)	X4	D4	0		17238	17121.9				*108946.09			***	428
IgG (77)	X5	E4	0		7437	7320.9				4838.07			4838.07	355
IgG (77)	X5	F4	0		7665	7548.9				5078.12			5078.12	426
IgG (77)	X6	G4	0		2914	2797.9				1358.91			1358.91	399
IgG (77)	X6	H4	0		2932	2815.9				1369.23			1369.23	329
IgG (77)	X7	A5	0		8437	8320.9				5960.55			5960.55	481
IgG (77)	X7	B5	0		8329.5	8213.4				5830.69			5830.69	428
IgG (77)	X8	C5	0		10994	10877.9				10004.87			10004.87	415
IgG (77)	X8	D5	0		10910	10793.9				9833.08			9833.08	349
IgG (77)	X9	E5	0		5769	5652.9				3309.69			3309.69	417
IgG (77)	X9	F5	0		6257.5	6141.4				3720.33			3720.33	384
IgG (77)	X10	G5	0		15049	14932.9				27808.75			27808.75	403
IgG (77)	X10	H5	0		15247.5	15131.4				29870.64			29870.64	414
IgG (77)	X11	A6	0		11569	11452.9				11284.01			11284.01	491
IgG (77)	X11	B6	0		11309	11192.9				10682.06			10682.06	589
IgG (77)	X12	C6	0		15024.5	14908.4				27572.09			27572.09	388
IgG (77)	X12	D6	0		14903	14786.9				26449.95			26449.95	419
Sampling Errors: 1 - Low bead #, 2 - Agg beads, 3 - Classify %, 4 - Region selection, 5 - Platform temperature
***Value not available / --- = Designated as an outlier
*Value extrapolated beyond standard range
OOR = Out of Range / OOR> = Out of Range Above  / OOR< = Out of Range Below
Exp Conc = Expected Concentration  /  Obs Conc = Observed  Concentration
Conc in Range = Unknown sample concentrations within range where standards recovery is 70-130%
Regression Type: Logistic - 5PL
Std. Curve: FI = -116.123 + (17703.3 + 116.123) / ((1 + (Conc / 100.442)^-1.26567))^0.694244
FitProb. = 0.6271, ResVar. = 0.4666